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mouse znf263 coding sequence  (Addgene inc)


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    Addgene inc mouse znf263 coding sequence
    (A) Venn diagram showing RAD21, CTCF, and PATZ1 binding in HEK293 cells. (B) Heat maps of RAD21, CTCF, PATZ1, MAZ, and other zinc finger proteins, <t>ZNF263,</t> ZNF341 and ZNF467 in HEK293 cells. ChIP-seq read density was grouped as Cluster 1, Cluster 2, and Cluster 3 based on the indicated overlaps with RAD21 signal within a 4 kb window. (C) Western blot analysis of RAD21, FLAG, and CTCF upon FLAG-PATZ1 immunoprecipitation from mESCs (n=2, see for biological replicate). (D) Visualization of Hi-C contact matrices for a zoomed-in region around the TBC1D1 locus in HepG2 cells. Shown below are loops with PATZ1 at both anchors in HepG2 cells, ChIP-seq read densities for RAD21, CTCF, PATZ1, and MAZ, and gene annotations. ChIP-seq data in HepG2 cells is from two combined biological replicates. (E) Percentage of Hi-C loops in HepG2 cells overlapping with RAD21, CTCF, and PATZ1 ChIP-seq peaks. (F) Heat maps of RAD21, PATZ1, ZNF263, ZNF341, and ZNF467 in HEK293 cells. ChIP-seq read density was grouped as Cluster 1, Cluster 2, Cluster 3, and Cluster 4 based on the combinatorial overlaps of zinc finger proteins with RAD21 within a 4 kb window in HEK293 cells. The model on the right side indicates combinatorial binding of the indicated factors in each cluster (see ). ChIP-seq data in HEK293 cells is from one replicate for RAD21 and one representative of two biological replicates for others (see for datasets).
    Mouse Znf263 Coding Sequence, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse znf263 coding sequence/product/Addgene inc
    Average 92 stars, based on 1 article reviews
    mouse znf263 coding sequence - by Bioz Stars, 2026-05
    92/100 stars

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    1) Product Images from "Members of an array of zinc finger proteins specify distinct Hox chromatin boundaries"

    Article Title: Members of an array of zinc finger proteins specify distinct Hox chromatin boundaries

    Journal: Molecular cell

    doi: 10.1016/j.molcel.2024.08.007

    (A) Venn diagram showing RAD21, CTCF, and PATZ1 binding in HEK293 cells. (B) Heat maps of RAD21, CTCF, PATZ1, MAZ, and other zinc finger proteins, ZNF263, ZNF341 and ZNF467 in HEK293 cells. ChIP-seq read density was grouped as Cluster 1, Cluster 2, and Cluster 3 based on the indicated overlaps with RAD21 signal within a 4 kb window. (C) Western blot analysis of RAD21, FLAG, and CTCF upon FLAG-PATZ1 immunoprecipitation from mESCs (n=2, see for biological replicate). (D) Visualization of Hi-C contact matrices for a zoomed-in region around the TBC1D1 locus in HepG2 cells. Shown below are loops with PATZ1 at both anchors in HepG2 cells, ChIP-seq read densities for RAD21, CTCF, PATZ1, and MAZ, and gene annotations. ChIP-seq data in HepG2 cells is from two combined biological replicates. (E) Percentage of Hi-C loops in HepG2 cells overlapping with RAD21, CTCF, and PATZ1 ChIP-seq peaks. (F) Heat maps of RAD21, PATZ1, ZNF263, ZNF341, and ZNF467 in HEK293 cells. ChIP-seq read density was grouped as Cluster 1, Cluster 2, Cluster 3, and Cluster 4 based on the combinatorial overlaps of zinc finger proteins with RAD21 within a 4 kb window in HEK293 cells. The model on the right side indicates combinatorial binding of the indicated factors in each cluster (see ). ChIP-seq data in HEK293 cells is from one replicate for RAD21 and one representative of two biological replicates for others (see for datasets).
    Figure Legend Snippet: (A) Venn diagram showing RAD21, CTCF, and PATZ1 binding in HEK293 cells. (B) Heat maps of RAD21, CTCF, PATZ1, MAZ, and other zinc finger proteins, ZNF263, ZNF341 and ZNF467 in HEK293 cells. ChIP-seq read density was grouped as Cluster 1, Cluster 2, and Cluster 3 based on the indicated overlaps with RAD21 signal within a 4 kb window. (C) Western blot analysis of RAD21, FLAG, and CTCF upon FLAG-PATZ1 immunoprecipitation from mESCs (n=2, see for biological replicate). (D) Visualization of Hi-C contact matrices for a zoomed-in region around the TBC1D1 locus in HepG2 cells. Shown below are loops with PATZ1 at both anchors in HepG2 cells, ChIP-seq read densities for RAD21, CTCF, PATZ1, and MAZ, and gene annotations. ChIP-seq data in HepG2 cells is from two combined biological replicates. (E) Percentage of Hi-C loops in HepG2 cells overlapping with RAD21, CTCF, and PATZ1 ChIP-seq peaks. (F) Heat maps of RAD21, PATZ1, ZNF263, ZNF341, and ZNF467 in HEK293 cells. ChIP-seq read density was grouped as Cluster 1, Cluster 2, Cluster 3, and Cluster 4 based on the combinatorial overlaps of zinc finger proteins with RAD21 within a 4 kb window in HEK293 cells. The model on the right side indicates combinatorial binding of the indicated factors in each cluster (see ). ChIP-seq data in HEK293 cells is from one replicate for RAD21 and one representative of two biological replicates for others (see for datasets).

    Techniques Used: Binding Assay, ChIP-sequencing, Western Blot, Immunoprecipitation, Hi-C

    (A) Normalized ChIP-seq densities for RAD21, CTCF, and PATZ1, and ZNF263 in WT, Patz1 KO, and Znf263 KO mESCs at the indicated regions in the HoxA cluster. ChIP-seq data represents one representative replicate of two biological replicates for RAD21, CTCF, and one replicate for FH-PATZ1 and FH-ZNF263. (B-D) RT-qPCR analysis for the indicated Hox genes in (B) the HoxA , (C) the HoxC , and (D) the HoxD clusters in WT and Patz1 KO cervical MNs. RT-qPCR signal was normalized to Atp5f1 and ActB levels. The fold-change in expression was calculated relative to WT MNs. All RT-qPCR results are represented as mean values and error bars indicating log 2 (SE) across three biological replicates (two-sided Student’s t -test without multiple testing correction; *** P ≤ 0.001, ** P ≤ 0.01, * P < 0.05). (E) Differentially expressed genes by RNA-seq upon Patz1 KO in MNs from two biological replicates (see all in ). (F) GO analysis showing the top biological processes enriched in the differentially expressed genes in Patz1 KO versus WT MNs. PANTHER overrepresentation test tools were used for GO analysis and top 15 categories having a fold enrichment > 2.5 were plotted (see all in ). (G-I) RT-qPCR analysis for the indicated Hox genes in (G) the HoxA , (H) the HoxC , and (I) the HoxD clusters in WT and Znf263 KO cervical MNs. RT-qPCR signal was normalized to Atp5f1 and Gapdh levels. The fold-change in expression was calculated relative to WT MNs. All RT-qPCR results are represented as mean values and error bars indicating log 2 (SE) across three technical replicates. Two independent Znf263 KO clones are shown (see ).
    Figure Legend Snippet: (A) Normalized ChIP-seq densities for RAD21, CTCF, and PATZ1, and ZNF263 in WT, Patz1 KO, and Znf263 KO mESCs at the indicated regions in the HoxA cluster. ChIP-seq data represents one representative replicate of two biological replicates for RAD21, CTCF, and one replicate for FH-PATZ1 and FH-ZNF263. (B-D) RT-qPCR analysis for the indicated Hox genes in (B) the HoxA , (C) the HoxC , and (D) the HoxD clusters in WT and Patz1 KO cervical MNs. RT-qPCR signal was normalized to Atp5f1 and ActB levels. The fold-change in expression was calculated relative to WT MNs. All RT-qPCR results are represented as mean values and error bars indicating log 2 (SE) across three biological replicates (two-sided Student’s t -test without multiple testing correction; *** P ≤ 0.001, ** P ≤ 0.01, * P < 0.05). (E) Differentially expressed genes by RNA-seq upon Patz1 KO in MNs from two biological replicates (see all in ). (F) GO analysis showing the top biological processes enriched in the differentially expressed genes in Patz1 KO versus WT MNs. PANTHER overrepresentation test tools were used for GO analysis and top 15 categories having a fold enrichment > 2.5 were plotted (see all in ). (G-I) RT-qPCR analysis for the indicated Hox genes in (G) the HoxA , (H) the HoxC , and (I) the HoxD clusters in WT and Znf263 KO cervical MNs. RT-qPCR signal was normalized to Atp5f1 and Gapdh levels. The fold-change in expression was calculated relative to WT MNs. All RT-qPCR results are represented as mean values and error bars indicating log 2 (SE) across three technical replicates. Two independent Znf263 KO clones are shown (see ).

    Techniques Used: ChIP-sequencing, Quantitative RT-PCR, Expressing, RNA Sequencing, Clone Assay

    Key Resources Table
    Figure Legend Snippet: Key Resources Table

    Techniques Used: Virus, Recombinant, Magnetic Beads, SYBR Green Assay, Western Blot, Cloning, Plasmid Preparation, Software, Injection



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    mouse znf263 coding sequence  (Addgene inc)
    92
    Addgene inc mouse znf263 coding sequence
    (A) Venn diagram showing RAD21, CTCF, and PATZ1 binding in HEK293 cells. (B) Heat maps of RAD21, CTCF, PATZ1, MAZ, and other zinc finger proteins, <t>ZNF263,</t> ZNF341 and ZNF467 in HEK293 cells. ChIP-seq read density was grouped as Cluster 1, Cluster 2, and Cluster 3 based on the indicated overlaps with RAD21 signal within a 4 kb window. (C) Western blot analysis of RAD21, FLAG, and CTCF upon FLAG-PATZ1 immunoprecipitation from mESCs (n=2, see for biological replicate). (D) Visualization of Hi-C contact matrices for a zoomed-in region around the TBC1D1 locus in HepG2 cells. Shown below are loops with PATZ1 at both anchors in HepG2 cells, ChIP-seq read densities for RAD21, CTCF, PATZ1, and MAZ, and gene annotations. ChIP-seq data in HepG2 cells is from two combined biological replicates. (E) Percentage of Hi-C loops in HepG2 cells overlapping with RAD21, CTCF, and PATZ1 ChIP-seq peaks. (F) Heat maps of RAD21, PATZ1, ZNF263, ZNF341, and ZNF467 in HEK293 cells. ChIP-seq read density was grouped as Cluster 1, Cluster 2, Cluster 3, and Cluster 4 based on the combinatorial overlaps of zinc finger proteins with RAD21 within a 4 kb window in HEK293 cells. The model on the right side indicates combinatorial binding of the indicated factors in each cluster (see ). ChIP-seq data in HEK293 cells is from one replicate for RAD21 and one representative of two biological replicates for others (see for datasets).
    Mouse Znf263 Coding Sequence, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse znf263 coding sequence/product/Addgene inc
    Average 92 stars, based on 1 article reviews
    mouse znf263 coding sequence - by Bioz Stars, 2026-05
    92/100 stars
    		  Buy from Supplier

    Image Search Results


    (A) Venn diagram showing RAD21, CTCF, and PATZ1 binding in HEK293 cells. (B) Heat maps of RAD21, CTCF, PATZ1, MAZ, and other zinc finger proteins, ZNF263, ZNF341 and ZNF467 in HEK293 cells. ChIP-seq read density was grouped as Cluster 1, Cluster 2, and Cluster 3 based on the indicated overlaps with RAD21 signal within a 4 kb window. (C) Western blot analysis of RAD21, FLAG, and CTCF upon FLAG-PATZ1 immunoprecipitation from mESCs (n=2, see for biological replicate). (D) Visualization of Hi-C contact matrices for a zoomed-in region around the TBC1D1 locus in HepG2 cells. Shown below are loops with PATZ1 at both anchors in HepG2 cells, ChIP-seq read densities for RAD21, CTCF, PATZ1, and MAZ, and gene annotations. ChIP-seq data in HepG2 cells is from two combined biological replicates. (E) Percentage of Hi-C loops in HepG2 cells overlapping with RAD21, CTCF, and PATZ1 ChIP-seq peaks. (F) Heat maps of RAD21, PATZ1, ZNF263, ZNF341, and ZNF467 in HEK293 cells. ChIP-seq read density was grouped as Cluster 1, Cluster 2, Cluster 3, and Cluster 4 based on the combinatorial overlaps of zinc finger proteins with RAD21 within a 4 kb window in HEK293 cells. The model on the right side indicates combinatorial binding of the indicated factors in each cluster (see ). ChIP-seq data in HEK293 cells is from one replicate for RAD21 and one representative of two biological replicates for others (see for datasets).

    Journal: Molecular cell

    Article Title: Members of an array of zinc finger proteins specify distinct Hox chromatin boundaries

    doi: 10.1016/j.molcel.2024.08.007

    Figure Lengend Snippet: (A) Venn diagram showing RAD21, CTCF, and PATZ1 binding in HEK293 cells. (B) Heat maps of RAD21, CTCF, PATZ1, MAZ, and other zinc finger proteins, ZNF263, ZNF341 and ZNF467 in HEK293 cells. ChIP-seq read density was grouped as Cluster 1, Cluster 2, and Cluster 3 based on the indicated overlaps with RAD21 signal within a 4 kb window. (C) Western blot analysis of RAD21, FLAG, and CTCF upon FLAG-PATZ1 immunoprecipitation from mESCs (n=2, see for biological replicate). (D) Visualization of Hi-C contact matrices for a zoomed-in region around the TBC1D1 locus in HepG2 cells. Shown below are loops with PATZ1 at both anchors in HepG2 cells, ChIP-seq read densities for RAD21, CTCF, PATZ1, and MAZ, and gene annotations. ChIP-seq data in HepG2 cells is from two combined biological replicates. (E) Percentage of Hi-C loops in HepG2 cells overlapping with RAD21, CTCF, and PATZ1 ChIP-seq peaks. (F) Heat maps of RAD21, PATZ1, ZNF263, ZNF341, and ZNF467 in HEK293 cells. ChIP-seq read density was grouped as Cluster 1, Cluster 2, Cluster 3, and Cluster 4 based on the combinatorial overlaps of zinc finger proteins with RAD21 within a 4 kb window in HEK293 cells. The model on the right side indicates combinatorial binding of the indicated factors in each cluster (see ). ChIP-seq data in HEK293 cells is from one replicate for RAD21 and one representative of two biological replicates for others (see for datasets).

    Article Snippet: The mouse Znf263 coding sequence (NM_148924.3) with an HA-tag sequence fused to its 5’ end was cloned into a PiggyBac vector (pPB-CAG-FLAG-HA, a modified version of pPB-CAG-3xFLAG-empty-pgk-hph from Addgene, #48754) to create pPB-CAG-FLAG-HA-ZNF263 plasmid via Gibson assembly (NEB, #E2611).

    Techniques: Binding Assay, ChIP-sequencing, Western Blot, Immunoprecipitation, Hi-C

    (A) Normalized ChIP-seq densities for RAD21, CTCF, and PATZ1, and ZNF263 in WT, Patz1 KO, and Znf263 KO mESCs at the indicated regions in the HoxA cluster. ChIP-seq data represents one representative replicate of two biological replicates for RAD21, CTCF, and one replicate for FH-PATZ1 and FH-ZNF263. (B-D) RT-qPCR analysis for the indicated Hox genes in (B) the HoxA , (C) the HoxC , and (D) the HoxD clusters in WT and Patz1 KO cervical MNs. RT-qPCR signal was normalized to Atp5f1 and ActB levels. The fold-change in expression was calculated relative to WT MNs. All RT-qPCR results are represented as mean values and error bars indicating log 2 (SE) across three biological replicates (two-sided Student’s t -test without multiple testing correction; *** P ≤ 0.001, ** P ≤ 0.01, * P < 0.05). (E) Differentially expressed genes by RNA-seq upon Patz1 KO in MNs from two biological replicates (see all in ). (F) GO analysis showing the top biological processes enriched in the differentially expressed genes in Patz1 KO versus WT MNs. PANTHER overrepresentation test tools were used for GO analysis and top 15 categories having a fold enrichment > 2.5 were plotted (see all in ). (G-I) RT-qPCR analysis for the indicated Hox genes in (G) the HoxA , (H) the HoxC , and (I) the HoxD clusters in WT and Znf263 KO cervical MNs. RT-qPCR signal was normalized to Atp5f1 and Gapdh levels. The fold-change in expression was calculated relative to WT MNs. All RT-qPCR results are represented as mean values and error bars indicating log 2 (SE) across three technical replicates. Two independent Znf263 KO clones are shown (see ).

    Journal: Molecular cell

    Article Title: Members of an array of zinc finger proteins specify distinct Hox chromatin boundaries

    doi: 10.1016/j.molcel.2024.08.007

    Figure Lengend Snippet: (A) Normalized ChIP-seq densities for RAD21, CTCF, and PATZ1, and ZNF263 in WT, Patz1 KO, and Znf263 KO mESCs at the indicated regions in the HoxA cluster. ChIP-seq data represents one representative replicate of two biological replicates for RAD21, CTCF, and one replicate for FH-PATZ1 and FH-ZNF263. (B-D) RT-qPCR analysis for the indicated Hox genes in (B) the HoxA , (C) the HoxC , and (D) the HoxD clusters in WT and Patz1 KO cervical MNs. RT-qPCR signal was normalized to Atp5f1 and ActB levels. The fold-change in expression was calculated relative to WT MNs. All RT-qPCR results are represented as mean values and error bars indicating log 2 (SE) across three biological replicates (two-sided Student’s t -test without multiple testing correction; *** P ≤ 0.001, ** P ≤ 0.01, * P < 0.05). (E) Differentially expressed genes by RNA-seq upon Patz1 KO in MNs from two biological replicates (see all in ). (F) GO analysis showing the top biological processes enriched in the differentially expressed genes in Patz1 KO versus WT MNs. PANTHER overrepresentation test tools were used for GO analysis and top 15 categories having a fold enrichment > 2.5 were plotted (see all in ). (G-I) RT-qPCR analysis for the indicated Hox genes in (G) the HoxA , (H) the HoxC , and (I) the HoxD clusters in WT and Znf263 KO cervical MNs. RT-qPCR signal was normalized to Atp5f1 and Gapdh levels. The fold-change in expression was calculated relative to WT MNs. All RT-qPCR results are represented as mean values and error bars indicating log 2 (SE) across three technical replicates. Two independent Znf263 KO clones are shown (see ).

    Article Snippet: The mouse Znf263 coding sequence (NM_148924.3) with an HA-tag sequence fused to its 5’ end was cloned into a PiggyBac vector (pPB-CAG-FLAG-HA, a modified version of pPB-CAG-3xFLAG-empty-pgk-hph from Addgene, #48754) to create pPB-CAG-FLAG-HA-ZNF263 plasmid via Gibson assembly (NEB, #E2611).

    Techniques: ChIP-sequencing, Quantitative RT-PCR, Expressing, RNA Sequencing, Clone Assay

    Key Resources Table

    Journal: Molecular cell

    Article Title: Members of an array of zinc finger proteins specify distinct Hox chromatin boundaries

    doi: 10.1016/j.molcel.2024.08.007

    Figure Lengend Snippet: Key Resources Table

    Article Snippet: The mouse Znf263 coding sequence (NM_148924.3) with an HA-tag sequence fused to its 5’ end was cloned into a PiggyBac vector (pPB-CAG-FLAG-HA, a modified version of pPB-CAG-3xFLAG-empty-pgk-hph from Addgene, #48754) to create pPB-CAG-FLAG-HA-ZNF263 plasmid via Gibson assembly (NEB, #E2611).

    Techniques: Virus, Recombinant, Magnetic Beads, SYBR Green Assay, Western Blot, Cloning, Plasmid Preparation, Software, Injection